SphI from New England Biolabs

Product Specs
ItemSphI
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0182L
Concentration5 u/µL
Quantity2500 units
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

GCATG/C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Gene duplication and deletion caused by over-replication at a fork barrierNature CommunicationsNovember 25, 2023Judith Oehler, Carl A. Morrow, Matthew C. Whitbyµl 10x ThermoPol buffer (New England Biolabs, M0267X), 0.6 µl 10 mM dNTPs (ThermoFisher Scientific, R0182); 0.6 µl 10 µM oMW1985, 0.6 µl 10 µM oMW1620/1628, and 0.15 µl of Taq DNA Polymerase (New England Biolabs,

(2) How Pol α-primase is targeted to replisomes to prime eukaryotic DNA replicationMolecular CellAugust 17, 2023Morgan L. Jones, Valentina Aria, Yasemin Baris, Joseph T.P. YeelesAM2616 Phusion® High-Fidelity DNA Polymerase,New England Biolabs,Cat# E0553 SphI,New England Biolabs,Cat# R0182 SapI,New England Biolabs,Cat# R0569 FspI,New England Biolabs,Cat# R0135 BsaHI,New England Biolabs,Cat#

(3) Biological features of TcM: A new Trypanosoma cruzi isolate from Argentina classified into TcV lineage✰Current Research in Microbial SciencesAugust 1, 2022Santiago José Martinez, Gonzalo Nicolás Nardella, Matías Exequiel Rodríguez, Cynthia Vanesa Rivero, Fernán Agüero, Patricia Silvia Romanoamplification products (832 bp) were simultaneously digested with 1 U of HpaI (NEB R0105) and 1 U of SphI (NEB R0182) for 1 h at 37 °C. Restriction fragments were then resolved in 2% TBE-agarose gel for 1 h at 5.33 V/cm.

(4) A Zika virus mutation enhances transmission potential and confers escape from protective dengue virus immunityCell ReportsApril 12, 2022Jose Angel Regla-Nava et al.Kit,Qiagen,Cat. #73404 AvrII,(New England BioLabs),Cat. #R0174S SphI,(New England BioLabs),Cat. #R0182S NaeI,(New England BioLabs),Cat. #R0190S ClaI,(New England BioLabs),Cat. #R0197S mMESSAGE mMACHINE™ T7 transcription kit

(5) Modelling Chlamydia and HPV co-infection in patient-derived ectocervix organoids reveals distinct cellular reprogrammingNature communicationsFebruary 24, 2022Koster S, Gurumurthy RK, Kumar N, Prakash PG, Dhanraj J, Bayer S, Berger H, Kurian SM, Drabkina M, Mollenkopf HJ, et al.µL forward primer (10 µM), 0.5 µl reverse primer (10 µM), 0.5 µl dNTPs (10 mM) (Thermo Scientific, #R0182), 2.5 µL 10x NEB buffer (BioLabs, #B9004S), 1 µL NEB Taq polymerase (5000 U/mL) (BioLabs, #M0267S),

(6) Mesmerize is a dynamically adaptable user-friendly analysis platform for 2D and 3D calcium imaging dataNature CommunicationsDecember 1, 2021Kushal Kolar, Daniel Dondorp, Jordi Cornelis Zwiggelaar, JÃ¸rgen HÃ¸yer, Marios ChatzigeorgiouPromega). Using purified gDNA at 150 ng/μl, the primers shown in Supplementary Table 5 dNTPs (Thermofisher, R0182) and the Q5 High-Fidelity DNA Polymerase (M0491L, NEB) we performed PCR reactions. The amplified PCR

(7) Dual RNA 3’-end processing of H2A.X messenger RNA maintains DNA damage repair throughout the cell cycleNature CommunicationsDecember 1, 2021Esther Griesbach, Margarita Schlackow, William F. Marzluff, Nick J. Proudfootpoly(A) site was excised using MluI and SphI restriction enzymes (New England Biolabs (NEB, #R0198S and #R0182S). The linearised plasmid was gel-purified using the Zymoclean Gel DNA Recovery Kit (Zymo Research, #D4007)

(8) Analysis of Drosophila Atg8 proteins reveals multiple lipidation-independent rolesAutophagySeptember 2, 2021András Jipa, Viktor Vedelek, Zsolt Merényi, Adél Ürmösi, Szabolcs Takáts, Attila L. Kovács, Gábor V. Horváth, Rita Sinka, Gábor Juhász3xHA-Atg8b-Atg8aUTR intermediate construct. pGen-3xHA-Atg8b-UTR plasmid was digested with SphI (NEB, R0182S)-EcoRI (NEB, R3101) enzymes and ligated with SphI (NEB, R0182S)-EcoRI (R0101S) digested Atg8a promoter

(9) Implication of ZNF217 in Accelerating Tumor Development and Therapeutically Targeting ZNF217-Induced PI3K–AKT Signaling for the Treatment of Metastatic OsteosarcomaMolecular Cancer TherapeuticsDecember 1, 2020Branden A. Smeester et al.generated via the LR cloning system (#11791020, Thermo Fisher) for #3 prior to linearization with SphI (#R0182, New England Biolabs) and pronuclear injection described in the following section. Pronuclear injection Transgenic

(10) Detection of Individual RNA in Fixed Cells and Tissues by Chromogenic ISHBIO-PROTOCOLFebruary 5, 2020Meng Jiang, Chen Lin, Rongqin Kecontaining PNK buffer A), store at -20 °C 21. dNTP Set, 100 mM Solutions (Thermo Scientific, catalog number: R0182), store at -20 °C 22. SplintR Ligase, 25 U/µl (NEB, catalog number: M0375L), store at -20 °C 23. phi29 DNA Polymerase

(11) Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease ModelsJournal of Visualized ExperimentsApril 16, 2019Lidia Tagliafierro, Ekaterina Ilich, Malik Moncalvo, Jeffrey Gu, Ahila Sriskanda, Carole Grenier, Susan K. Murphy, Ornit Chiba-Falek, Boris KantorR0195S KpnI New England Biolabs R0142S PacI New England Biolabs R0547S SphI New England Biolabs R0182S Materials Company Catalog Number Comments

(12) DNA content analysis allows discrimination between Trypanosoma cruzi and Trypanosoma rangeliPLOS ONEDecember 19, 2017Lucila Langoni Naves, Marcos Vinícius da Silva, Emanuella Francisco Fajardo, Raíssa Bernardes da Silva, Fernanda Bernadelli De Vito, Virmondes Rodrigues, Eliane Lages-Silva, Luis Eduardo Ramírez, André Luiz Pedrosadigested with 1U of the enzyme HpaI (NEB R105) at 55˚C for 1 h and with 1U of the enzyme SphI (NEB R0182) at 37˚C for 1 h (TcSC5D fragment) or with one unit of XhoI (NEB R0146S) endonuclease (TcMK fragment). The

(13) Ciliary transcription factors and miRNAs precisely regulate Cp110 levels required for ciliary adhesions and ciliogenesiseLifeSeptember 13, 2016Walentek P, Quigley IK, Sun DI, Sajjan UK, Kintner C, Harland RM.and subcloned into the pCS107 expression vector, which was digested with Sph1 (New England Biolabs, #R0182S) and re-ligated to remove the miR binding site, as previously described (Song et al., 2014). DNAs were

(14) miR-34/449 miRNAs are required for motile ciliogenesis by repressing cp110NatureJune 5, 2014Song R, Walentek P, Sponer N, Klimke A, Lee JS, Dixon G, Harland R, Wan Y, Lishko P, Lize M, et al.Cp110Δ3′UTR was generated from the same clone, which was digested with Sph1 (New England Biolabs, #R0182S) and re-ligated to remove most of the 3′UTR. Analysis of morphant tadpole brains for signs of hydrocephalus

(15) A Simple Strain Typing Assay for Trypanosoma cruzi: Discrimination of Major Evolutionary Lineages from a Single Amplification ProductPLoS Neglected Tropical DiseasesJuly 31, 2012Raul O. Cosentino, Fernán Agüeroamplification products where digested in a single incubation with 1 U of HpaI (NEB R0105) and 1 U SphI (NEB R0182) at 37 for 1 h. Digestion was performed in the same buffer used for PCR amplification (Invitrogen Taq

(16) Fitness analyses of all possible point mutations for regions of genes in yeastNature ProtocolsJuly 1, 2012Ryan Hietpas, Benjamin Roscoe, Li Jiang, Daniel N. A. Bolonendonuclease (New England Biolabs, cat. no.R0535) SphI restriction endonuclease (New Englan Biolabs, cat. no.R0182) MmeI restriction endonuclease (New England Biolabs, cat. no. R0637L) S-adenosyl methionine (SAM;

(17) An efficient method of directly cloning chimpanzee adenovirus as a vaccine vectorNature ProtocolsNovember 1, 2010Dongming Zhou, Xiangyang Zhou, Ang Bian, Hua Li, Heng Chen, Juliana C Small, Yan Li, Wynetta Giles-Davis, Zhiquan Xiang, Hildegund C J Ertlcat. no. R0130S) NdeI (New England Biolabs, cat. no. R0111S) SphI (New England Biolabs, cat. no. R0182S) HindIII (New England Biolabs, cat. no. R0104S) AgeI (New England Biolabs, cat. no. R0552S) MluI

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SphI

SphI from New England Biolabs

Sequence

New England Biolabs

Citations (17)

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SphI from New England Biolabs

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